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c‐ fos gene expression in cell revertants from a transformed to a pseudonormal phenotype
Author(s) -
Lavrovsky Y.V.,
Svinarchuk F.P.,
Lavrovsky V.A.,
Yefremov Y.R.,
Vlassov V.V.
Publication year - 1993
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(93)81207-g
Subject(s) - chloramphenicol acetyltransferase , c fos , phenotype , microbiology and biotechnology , gene , transcription (linguistics) , biology , gene expression , cell , messenger rna , promoter , genetics , linguistics , philosophy
c‐ fos gene expression in two types of mouse sarcoma cells of spontaneous origin and in revertants to pseudonormal phenotype has been investigated. In the latter cells the content of c‐ fos mRNA is similar to that in normal fibroblasts. Activity of transcription factors interacting with the regulatory elements, SRE, DSE and TRE, in the c‐ fos promoter do not correlate with the c‐ fos mRNA concentration. However, experiments with cells transformed with the indicator plasmid, fos ‐CAT, showed that the 600 bp c‐fos promoter region provides the chloramphenicol acetyltransferase activity correlating with c‐ fos mRNA expression in cell revertants to a pseudonormal phenotype.