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Identification and characterization of an anti‐tyrosine kinase factor in cystic gliomas
Author(s) -
Berger F.,
Laine M.,
Amalfitano G.,
Brunet J.F.,
Verna J.M.,
Nissou M.F.,
Benabid A.L.
Publication year - 1993
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(93)81204-d
Subject(s) - epidermal growth factor , autocrine signalling , tyrosine kinase , cancer research , tyrosine phosphorylation , receptor tyrosine kinase , biology , growth factor , epidermal growth factor receptor , phosphorylation , carcinogenesis , a431 cells , signal transduction , medicine , microbiology and biotechnology , receptor , biochemistry , oncogene , gene , cell cycle
In view of the frequent activation of the epidermal growth factor receptor (EGF‐R) in gliomas and autocrine hypothesis, we searched for ‘EGF‐like’ factor(s) in cystic fluids (CFs) associated with gliomas. Membranes of A431 cells, which overexpress EGF‐R, were used to explore such activity in 20 CFs. In all cases CFs induced inhibition of EGF‐R phosphorylation. Biochemical analysis revealed an anti‐tyrosine kinase activity which was identified as a 18 kDa proteic factor. Effectiveness at high dilution and anti‐proliferative effect on living cells in culture suggest that this factor may be involved in the negative regulation of glial oncogenesis.