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Lipid transfer proteins (nsLTPs) from barley and maize leaves are potent inhibitors of bacterial and fungal plant pathogens
Author(s) -
Molina Antonio,
Segura Ana,
García-Olmedo Francisco
Publication year - 1993
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(93)81198-9
Subject(s) - plant lipid transfer proteins , biochemistry , clavibacter michiganensis , biology , pseudomonas syringae , escherichia coli , microbiology and biotechnology , pathogen , chemistry , gene
Four homogeneous proteins (Cw 18 , Cw 20 , Cw 21 , Cw 22 ) were isolated from etiolated barley leaves by extraction of the insoluble pellet from a Tris‐HCl (pH 7.5) homogenate with 1.5 M LiCl and fractionation by reverse‐phase high‐performance liquid chromatography. All 4 proteins inhibited growth of the pathogen Clavibacter michiganensis subsp. sepedonicus (EC 50 S = 1−3 × 10 −7 M) and had closely related N‐terminal amino acid sequences. The complete amino acid sequences of proteins Cw 18 and Cw 21 were determined and found to be homologous to previously described, non‐specific lipid transfer proteins from plants (32–62% identical positions). The proteins also inhibited growth of the bacterial pathogen Pseudomonas solanacearum (EC 50 s = 3–6 × 10 −7 M) and the fungus Furium solani (EC 50 s = 3–20 × 10 −6 M). A homologous protein from maize leaves (Cw 41 ) was purified in a similar manner and also found to have inhibitory properties, A synergistic effect against the fungus was observed when protein Cw 21 , was combined with thionins. A defense role for non‐specific lipid transfer proteins from plants is proposed.