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Activation of muscarinic receptors increases the concentration of free Na + in mouse pancreatic B‐cells
Author(s) -
Gilon P.,
Henquin J.C.
Publication year - 1993
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(93)81193-4
Subject(s) - muscarinic acetylcholine receptor , depolarization , extracellular , chemistry , tetrodotoxin , acetylcholine , biophysics , ouabain , endocrinology , receptor , muscarinic acetylcholine receptor m1 , medicine , muscarinic acetylcholine receptor m3 , membrane potential , sodium , biology , biochemistry , organic chemistry
The fluorescent probe SBFI was used to monitor the influence of acetylcholine (ACh) on the cytosolic concentration of free Na + (Na + i ) in single mouse pancreatic B‐cells. In the presence of 3 mM glucose and 135 mM extracellular Na + , Na + i , averaged 16.6 mM. ACh (100μM) increased Na + i by ~ 80%. This rise was prevented by atropine, a blocker of muscarinic receptors, and by omission of extracellular Na + , but still occurred if the sodium pump was blocked by ouabain. It was unaffected by tetrodotoxin, a blocker of voltage‐sensitive Na + channels, and was not mimicked by depolarization of the cells with high K + It is concluded that activation of muscarinic receptors increases the membrane permeability to Na + in pancreatic B‐cells.