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Activation of phosphatidylinositol lipid‐specific phospholipase C‐β 3 by G‐protein βγ subunits
Author(s) -
Carozzi Amanda,
Camps Montserrat,
Gierschik Peter,
Parker Peter J.
Publication year - 1993
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(93)81190-b
Subject(s) - heterotrimeric g protein , transducin , phospholipase c , g protein , gq alpha subunit , protein subunit , phosphatidylinositol , gtp' , chemistry , biochemistry , gtp binding protein regulators , phospholipase , g alpha subunit , inositol , microbiology and biotechnology , receptor , biology , signal transduction , enzyme , gene
A novel member of the inositol lipid‐specific phospholipase C family, PtdIns‐PLCβ 3 , is shown to be activated by βγ subunits of the heterotrimeric GTP‐binding protein, transducin. The activation is a direct effect since it is observed with the purified proteins. Furthermore, the activation is blocked by the GDP‐liganded α subunit of transducin, confirming that the effect is due to free βγ subunits. The implications with respect to receptor‐PtdIns‐PLC coupling are discussed.