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Cyclosporin A suppression of uncoupling in liver mitochondria of ground squirrel during arousal from hibernation
Author(s) -
Brustovetsky N.N.,
Egorova M.V.,
Gnutov D.Yu.,
Mokhova E.N.,
Skulachev V.P.
Publication year - 1993
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(93)81170-5
Subject(s) - ground squirrel , oligomycin , hibernation (computing) , mitochondrion , respiration , uncoupling agents , arousal , incubation , chemistry , biology , membrane potential , endocrinology , medicine , biophysics , microbiology and biotechnology , biochemistry , thermoregulation , anatomy , state (computer science) , atpase , algorithm , neuroscience , computer science , enzyme
Energy coupling parameters were studied in liver mitochondria of ground squirrel during arousal from hibernation. It was found that such mitochondria become uncoupled during incubation with phosphate in a salt medium. The uncoupling was revealed by respiration rate increase and membrane potential decrease in the presence of oligomycin. Both effects were reversed by addition of cyclosporin A. Under the same in vitro conditions, mitochondria from aroused (active) animals showed no uncoupling but could be uncoupled by addition of palmitate in the cyclosporin A‐sensitive fashion. It is proposed that formation of cyclosporin A‐sensitive pores can be involved in urgent heat production in arousing hibernators.