Premium
Regulation of Cu,Zn‐ and Mn‐superoxide dismutase transcription in Saccharomyces cerevisiae
Author(s) -
Galiazzo Francesca,
Labbe-Bois Rosine
Publication year - 1993
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(93)81162-s
Subject(s) - derepression , sod2 , superoxide dismutase , paraquat , sod1 , chemistry , dismutase , microbiology and biotechnology , biochemistry , catalase , saccharomyces cerevisiae , reactive oxygen species , gene expression , biology , yeast , oxidative stress , gene , psychological repression
The regulation of Cu,Zn‐ and Mn‐superoxide dismutases (SOD) was investigated by Northern blotting and gene fusions of SOD1 and S0D2 promoters with the β‐galactosidase reporter gene. Cu,ZnSOD expression was increased 3‐fold under glucose derepressing conditions, and decreased 4‐ to 6‐fold by oxygen or heme deficiency. MnSOD expression was increased 5‐fold by glucose derepression, and decreased 8‐ to 10‐fold by anaerobiosis and 4‐ to 5‐fold by heme deficiency. Induction by paraquat was modest, about 50% for SOD1 and 100% for SOD2 ; it was apparently independent of the respiratory chain function.