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Oxytocin induced cAMP‐dependent protein kinase activation and urokinase‐type plasminogen activator production in LLC‐PK 1 renal epithelial cells is mediated by the vasopressin V 2 ‐receptor
Author(s) -
Jans David A.,
Pavo Imre,
Fahrenholz Falk
Publication year - 1993
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(93)81149-t
Subject(s) - plasminogen activator , activator (genetics) , vasopressin , chemistry , receptor , endocrinology , oxytocin , medicine , urokinase , urokinase receptor , microbiology and biotechnology , biology , biochemistry
Using a variety of peptide analogues of oxytocin (OT) and Arg 8 ‐vasopressin (AVP), OT‐mediated induction of urokinase‐type plasminogen activator (uPA) was examined in LLC‐PK 1 renal epithelial cells, which possess distinct high‐affinity receptors of both the OT‐ and vasopressin renal (V 2 ‐) types. OT or OT‐receptor specific agonists induced concentration‐dependent cAMP synthesis, activation of the cAMP‐dependent protein kinase (cAMP‐PK) and uPA production consistent with their respective binding affinities for the V 2 ‐ and not the OT‐receptor. OT‐mediated uPA induction could be inhibited in a concentration‐dependent fashion by coincubation with a V 2 /V 1 ‐receptor specific antagonist, but not by an OT‐receptor specific antagonist. Results implied that stimulation of cAMP‐ and uPA responses in LLC‐PK 1 cells by OT was V 2 ‐receptor‐mediated.