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A rat brain cDNA encoding the neurotransmitter transporter with an unusual structure
Author(s) -
Liu Qing-Rong,
Mandiyan Sreekala,
López-Corcuera Beatriz,
Nelson Hannah,
Nelson Nathan
Publication year - 1993
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(93)81145-p
Subject(s) - neurotransmitter transporter , neurotransmitter , transporter , complementary dna , neurotransmitter systems , encoding (memory) , chemistry , neuroscience , biology , gene , biochemistry , dopamine , central nervous system
A rat cDNA clone encoding the novel membrane protein of the neurotransmitter transporters family was cloned and sequenced. The cDNA was identified as a transcript of the gene NTT4 of which a partial genomic clone was previously sequenced. Alignment of the amino acid sequence of NTT4 with other members of the neurotransmitter transport family revealed a marked deviattion from the conserved structure of all other members of the family. The largest extracellular loop with a potential glycosylation site was identified between membrane segments 7 and 8. The protein retains the common glycosylated loop between transmembrane helices 3 and 4 in all members of the family. The transcript of NTT4 was found exclusively in the central nervous system and is more abundant in the cerebellum and the cerebral cortex.