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Disulfide bridges in human complement component C3b
Author(s) -
Dolmer Klavs,
Sottrup-Jensen Lars
Publication year - 1993
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(93)81139-q
Subject(s) - disulfide bond , complement (music) , chemistry , residue (chemistry) , component (thermodynamics) , stereochemistry , biochemistry , physics , complementation , gene , phenotype , thermodynamics
The disulfide bridges of human complement component C3b, derived from C3 by removal of the 77‐residue C3a, have been determined. The 10 bridges are Cys 537 ‐Cys 794 , Cys 605 ‐Cys 640 , Cys 851 ‐Cys 1491 , Cys 1079 ‐Cys 1169 , Cys 1336 ‐Cys 1467 , Cys 1367 ‐Cys 1436 , Cys 1448 ‐Cys 1489 , Cys 1496 ‐Cys 1568 , Cys 1515 ‐Cys 1639 , and Cys 1615 ‐Cys 1624 . Including the 3 bridges in C3a (Cys 670 ‐Cys 698 , Cys 672 ‐Cys 705 , and Cys 685 ‐Cys 706 ) previously determined by high‐resolution X‐ray crystallography [Hoppe‐Seyler's Z. Physiol. Chem. 361 (1980) 1389‐1399] all disulfide bridges of C3 are localized. C3 and the strongly related C4 and C5 are members of the α 2 ‐macroglobulin superfamily. The predicted bridge patterns of C4 and C5 are discussed and compared with that of α 2 ‐macroglobulin.