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Identification of toxigenic Vibrio cholerae from the Argentine outbreak by PCR for ctx A1 and ctx A2‐B
Author(s) -
Varela Pablo,
Rivas Marta,
Binsztein Norma,
Cremona Maria Laura,
Herrmann Patricio,
Burrone Oscar,
Ugalde Rodolfo A.,
Frasch Alberto C.C.
Publication year - 1993
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(93)81136-n
Subject(s) - vibrio cholerae , cholera toxin , polymerase chain reaction , microbiology and biotechnology , biology , enterotoxin , operon , virology , toxin , outbreak , vibrionaceae , gene , escherichia coli , bacteria , genetics
A polymerase chain reaction (PCR) to detect a region of the A1 cholera toxin gene was applied to the identification of 43 Vibrio cholerae strains isolated from the recent outbreak in Argentina. A good correlation was observed between the GM1‐enzyme‐linked immunosorbent assay (GM1‐ELISA) to detect the B subunit of the enterotoxin and PCR. However, a V. cholerae non‐01 strain that was negative by the ELISA test, was positive by the PCR assay for the Al region. A second PCR test to detect the A2‐B coding region was developed to solve this case. We propose that routine detection of toxigenic V. cholerae by PCR should include analysis of A2‐B coding region or the whole cholera toxin operon.