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Selective cleavage of 28S rRNA variable regions V3 and V13 in myeloid leukemia cell apoptosis
Author(s) -
Houge Gunnar,
Døskeland Stein O.,
Bøe Roald,
Lanotte Michel
Publication year - 1993
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(93)81123-h
Subject(s) - myeloid leukemia , cleavage (geology) , apoptosis , leukemia , chemistry , biology , cancer research , biochemistry , genetics , paleontology , fracture (geology)
Vigorous apoptosis is induced 3–4 hours after activation of cAMP‐dependent protein kinase I in the rat myeloid leukemia cell line IPC‐81 [J. Cell. Physiol. 146 (1991) 73‐80]. We will report a novel feature of apoptosis in these cells: a selective and temporarily ordered cleavage within the two largest 28S ribosomal RNA variable regions (V3 and V13). The cleavage of 28S rRNA coincided with internucleosomal DNA fragmentation and cessation of cellular protein synthesis. The implication of 28S variable regions as targets in apoptosis is a clue to the function of these so far apparently superfluous parts of eukaryotic ribosomes.