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Procathepsin L‐specific antibodies that recognize procathepsin L but not cathepsin L
Author(s) -
Ishidoh Kazumi,
Takeda-Ezaki Mitsue,
Kominami Eiki
Publication year - 1993
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(93)81116-h
Subject(s) - antibody , chemistry , biochemistry , microbiology and biotechnology , biology , immunology
Procathepsin L was purified to apparent homogeneity from the culture medium of v‐Ha‐ras transformed NIH3T3 (Ras‐NIH) cells in three steps; anion‐exchange chromatography, gel nitration, and re‐gel filtration. SDS‐PAGE analyses revealed that the purified samples contained only the precursor form, procathepsin L, but not the mature enzyme, cathepsin L. Antibodies against purified procathepsin L were raised. These recognized both rat cathepsin L and the purified procathepsin L. To isolate procathepsin L‐specific antibodies that did not recognize cathepsin L, sequential affinity chromatography procedures were carried out. Immunoblot analyses showed that the procathepsin L‐specific antibodies recognized only procathepsin L, but not cathepsin L.