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Chaperonins dependent increase of Cu,Zn Superoxide dismutase production in Escherichia coli
Author(s) -
Battistoni Andrea,
Carrí M.Teresa,
Steinkühler Christian,
Rotilio Giuseppe
Publication year - 1993
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(93)81099-l
Subject(s) - groel , groes , chaperonin , escherichia coli , superoxide dismutase , enzyme , dismutase , chemistry , biochemistry , yield (engineering) , microbiology and biotechnology , protein folding , biology , materials science , gene , metallurgy
Over‐expression of the chaperonins GroEL and GroES significantly suppressed the temperature‐dependent pattern of expression of Cu,Zn Superoxide dismutases in Escherichia coli and increased the yield of active enzyme. The results obtained indicate that chaperonins prevent degradation of metal‐deficient enzyme molecules. GroEL was shown to form a complex with unfolded Cu,Zn Superoxide dismutase in vitro, confirming that GroEL can interact with β‐stranded proteins.