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Molecular action of the type 1 ribosome‐inactivating protein saporin 5 on Vicia sativa ribosomes
Author(s) -
Iglesias Rosario,
Arias F.Javier,
Rojo M.Angeles,
Escarmis Cristina,
Ferreras J.Miguel,
Girbés Tomás
Publication year - 1993
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(93)81091-d
Subject(s) - saporin , ribosome , ribosome inactivating protein , vicia sativa , protein biosynthesis , chemistry , translation (biology) , action (physics) , biology , biochemistry , botany , rna , messenger rna , in vitro , physics , immunotoxin , cytotoxicity , gene , quantum mechanics
The type 1 ribosome‐inactivating protein (RIP) saporin 5 isolated from seeds of Saponaria officinalis L. strongly inhibited translation carried out by Vicia sativa L. purified ribosomes. The toxin multidepurinated V. sativa rRNA, which upon treatment with acid aniline releases several RNA fragments including an RNA fragment of approximately 370 nucleotides the 5'‐end sequence of which was 5'‐GAGGAACG‐3'.