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Pre‐translational regulation of lipid synthesizing enzymes and surfactant proteins in fetal rat lung in explant culture
Author(s) -
Fraslon C.,
Batenburg J.J.
Publication year - 1993
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(93)81090-m
Subject(s) - phosphocholine , malic enzyme , in vivo , biochemistry , explant culture , messenger rna , enzyme , fatty acid synthase , fatty acid synthesis , fatty acid , biology , atp citrate lyase , chemistry , microbiology and biotechnology , in vitro , phospholipid , citrate synthase , dehydrogenase , phosphatidylcholine , gene , membrane
In hormone‐free explant cultures of 18‐day fetal rat lung the levels of the mRNAs for fatty acid synthase, ATP citrate lyase and surfactant proteins A, B, and C, increased as they do in vivo. Also CTP:phosphocholine cytidylyltransferase mRNA increased spontaneously. Unlike in vivo, malic enzyme mRNA increased drastically in cultured explants. Culture with dexamethasone increased the abundance of fatty acid synthase and surfactant protein mRNAs, but considerably depressed that of malic enzyme mRNA. Dexamethasone had little effect on CTP:phosphocholine cytidylyltransferase mRNA, supporting the concept that the increased activity of this enzyme caused by glucocorticoid is due to increased fatty acid synthesis.