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The genes aroA and trnQ are located upstream of psbO in the chromosome of Synechocystis 6803
Author(s) -
Mayes Steve R.,
Chiesa Marta Dalla,
Zhang Zhihong,
Barber James
Publication year - 1993
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(93)81084-d
Subject(s) - aroa , upstream (networking) , chromosome , gene , biology , genetics , upstream and downstream (dna) , synechocystis , escherichia coli , computer science , enterobacteriaceae , mutant , computer network
We have identified the existence of two genes, trnQ and aroA , located upstream of the psbO gene in Synechocystis sp. PCC 6803. The trnQ gene encodes a glutamine‐specific transfer RNA (tRNA Gln ) and the sequence given is the first reported for any cyanobacterium. The gene seems to exist as a single copy since its deletion results in nonviable mutation. The aroA gene encodes for 5‐enolpyruvylshikimate 3‐phosphate synthase and its discovery in the genome of Synechocystis 6803 is the first genetic evidence for the existence of the shikimate biosynthetic pathway in cyanobacteria. Interestingly, the partial sequence shares close homologies with the sequences of aroA from Gram‐positive bacteria.