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Identification of a nuclear protein binding element within the rat brain protein kinase C γ promoter that is related to the developmental control of this gene
Author(s) -
Chen Kuang-Hua,
Widen Steven G.,
Wilson Samuel H.,
Huang Kuo-Ping
Publication year - 1993
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(93)81075-b
Subject(s) - protein kinase c , nuclear protein , microbiology and biotechnology , biology , gene , isozyme , binding protein , electrophoretic mobility shift assay , protein kinase a , kinase , binding site , footprinting , transcription factor , biochemistry , enzyme
Protein kinase C γ (PKC γ) is a brain‐specific isozyme expressed at a high level in the adult but not in the fetal or newborn rat. At least seventeen nuclear protein binding sites within the 5'‐flanking region extending from −1612 to +243 had been identified by DNase I footprinting analysis and gel mobility shift assays. Among them, one site, GAATTAATAGG, at −669 to −679 is protected from DNase I digestion by nuclear protein from newborn but not from the adult rat brain. The levels of this binding protein, as determined by gel mobility shift assay, were found inversely related to the levels of PKC γ in rat brain at different stages of development. These results suggest that this particular binding site may participate in the developmental regulation of PKC γ gene.