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Annexin 1 is present in different molecular forms in rat cerebral cortex
Author(s) -
Pradel L.A.,
Rendon A.
Publication year - 1993
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(93)81035-x
Subject(s) - synaptophysin , synaptic vesicle , annexin , cerebral cortex , annexin a2 , biochemistry , cortex (anatomy) , membrane , chemistry , synaptic membrane , microbiology and biotechnology , biology , vesicle , neuroscience , immunohistochemistry , in vitro , immunology
Previously we have purified annexin 1 [J. Neurochem. 56 (1991) 1985‐1986] from pig cerebral cortex as a monomeric protein of 37 kDa. Here, the localization of annexin 1 was investigated in subcellular fractionations of rat cerebral cortex using immunodetection by a specific antibody. In contrast to synaptophysin, a specific synaptic vesicle integral membrane protein, annexin 1 is located in the synaptic plasma membrane fraction where it appears on SDS‐PAGE as a polypeptide of 74 kDa. Annexin 1 is extracted also as a 74 kDa polypeptide from the purified synaptic plasma membranes. These results suggest for the 74 kDa molecular form an enzymatic dimerization of annexin 1 when associated to the membrane.