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The existence of pre‐mature 16S rRNA species in plastid ribosomes
Author(s) -
Vera Antonio,
Yokoi Fumiaki,
Sugiura Masahiro
Publication year - 1993
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(93)81032-u
Subject(s) - plastid , ribosome , ribosomal rna , rnase p , biology , 16s ribosomal rna , nucleotide , nicotiana tabacum , biochemistry , bacteria , genetics , rna , chloroplast , gene
Plastid 70S ribosomes were prepared from heterotrophic cultured cells of tobacco ( Nicotiana tabacum , BY2), and the 5' termini of the 16S rRNA molecules present in the ribosomes were analyzed. RNase protection and primer extension experiments showed that a minor fraction of the 16S rRNA species carries a leader sequence of 30 nucleotides, coinciding with a putative RNase III cleavage site. The results suggest that an RNase Ill‐like activity is present in plastids and that ultimate 5' maturation of 16S rRNA takes place within the ribosome.