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The CRE‐binding factor ATF‐2 facilitates the occupation of the CCAAT box in the fibronectin gene promoter
Author(s) -
Srebrow Anabella,
Muro Andres F.,
Werbajh Santiago,
Sharp Phillip A.,
Kornblihtt Alberto R.
Publication year - 1993
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(93)81031-t
Subject(s) - caat box , microbiology and biotechnology , promoter , transcription factor , gene , biology , binding site , dna binding protein , transcription (linguistics) , fibronectin , gene expression , genetics , cell , linguistics , philosophy
The cAMP response element (CRE) and the CCAAT box of the fibronectin gene promoter are separated by only twenty base pairs. A specific factor that binds the CRE interacts cooperatively with the protein which binds to the adjacent CCAAT box, stimulating transcription [1992, J. Biol. Chem. 267, 12767‐12774]. Here we show that the CRE factor is an heterodimer between a 43 kDa and the ‘73 kDa’ CRE‐binding proteins and we identify the latter as ATF‐2 (also named CRE‐BPI), a protein implicated in recruiting transcriptional activators to promoters, able to form heterodimers with Jun and for which a sequence‐deduced MW of 55 kDa had been previously reported.