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Phorbol ester markedly increases the sensitivity of intestinal epithelial cells to 1,25‐dihydroxyvitamin D 3
Author(s) -
Armbrecht H.James,
Hodam Tara L.,
Boltz Monica A.,
Chen Ming L.
Publication year - 1993
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(93)81028-x
Subject(s) - phorbol ester , chemistry , in vitro , messenger rna , microbiology and biotechnology , endocrinology , medicine , mechanism of action , cytochrome p450 , cell culture , phorbol , enzyme , biology , biochemistry , protein kinase c , gene , genetics
We have used a clonal intestinal epithelial cell line (IEC‐18) to study the mechanism of action of 1,25‐dihydroxyvitamin D 3 (1,25(OH) 2 D) in vitro. 1,25(OH) 2 D (10 −7 M) elevated by over 10‐fold the mRNA levels for the cytochrome P450 component (P450cc24) of the 1,25(OH) 2 D‐24‐hydroxylase. Increased P450cc24 mRNA levels were detectable at 6 h and peaked at 36 h. Below a concentration of 10 −7 M, 1,25(OH) 2 D had almost no effect. However, addition of phorbol ester for 2 h made the intestine responsive to 1,25(OH) 2 D concentrations as low as 10 −9 M.