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Possible regulation of CFTR‐chloride channels by membrane‐bound phosphatases in pancreatic duct cells
Author(s) -
Becq F.,
Fanjul M.,
Merten M.,
Figarella C.,
Hollande E.,
Gola M.
Publication year - 1993
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(93)81016-s
Subject(s) - chloride channel , apical membrane , phosphatase , chemistry , ibmx , alkaline phosphatase , okadaic acid , microbiology and biotechnology , forskolin , theophylline , transfection , biochemistry , membrane , endocrinology , biology , enzyme , receptor , gene
We have studied CFTR‐Cl − channels in non‐CF CAPAN‐1 and in CFTR‐transfected CFPAC‐PLJ‐CFTR‐6 epithelial cells from human pancreas. Theophylline and IBMX induced the opening of cell‐attached CFTR‐Cl − channels. Theophylline, IBMX and the alkaline phosphatase (AP) inhibitor levamisole enhanced the activity of excised channels and reduced by 70–75% the apical membrane‐associated APs activity. Okadaic acid had no effect on APs and channel activities. A polyclonal anti‐alkaline phosphatase antibody (which detected apical APs) reduced APs activity and activated quiescent excised chloride channels. These results suggest that CFTR channels may be regulated by membrane‐bound phosphatases.