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Primary structures and expression from cDNAs of rat opioid receptor δ‐and μ‐subtypes
Author(s) -
Fukuda Kazuhiko,
Kato Shigehisa,
Mori Kenjiro,
Nishi Miyuki,
Takeshima Hiroshi
Publication year - 1993
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(93)81011-n
Subject(s) - chinese hamster ovary cell , microbiology and biotechnology , cloning (programming) , opioid receptor , biology , receptor , orphan receptor , messenger rna , molecular cloning , complementary dna , gene , opioid , genetics , computer science , transcription factor , programming language
The complete amino acid sequences of rat opioid receptors (designated as ROR‐A and ROR‐B) have been deduced by cloning and sequencing the cDNAs. The ligand‐binding properties of ROR‐A and ROR‐B expressed from the cloned cDNAs in Chinese hamster ovary cells correspond most closely to those of the pharmacologically defined δ‐ and μ‐opioid receptor subtypes, respectively. RNA blot hybridization analysis revealed that cerebrum and brainstem contain both ROR‐A and ROR‐B mRNAs, whereas neither ROR‐A nor ROR‐B mRNAs can be detected in cerebellum.