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Glutathione, but not glutamine, is detected in 13 C‐NMR spectra of perchloric acid extracts from C6 glioma cells
Author(s) -
Portais Jean-Charles,
Martin Magali,
Canioni Paul,
Merle Michel
Publication year - 1993
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(93)81009-o
Subject(s) - perchloric acid , chemistry , glutathione , glutamine , glioma , spectral line , nmr spectra database , nuclear magnetic resonance , radiochemistry , biochemistry , amino acid , inorganic chemistry , biology , cancer research , physics , enzyme , astronomy
Glutamine, which is expected to be produced by C6 glioma cells, is not detected in both amino‐acid analyses and 13 C‐NMR spectra of perchloric acid extracts of cells incubated for 4 h with [1‐ 13 C]glucose in the absence of extracellular glutamine. However, the resonances of a glutamate‐linked product are observed in these spectra. The analysis of the pH dependence of chemical shifts from various glutamate‐derived compounds shows that the observed resonances came from glutathione. Glutamine and glutathione signals are in close proximity on the frequency scale, leading to possible misinterpretation of the spectra.