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Mature carnitine palmitoyltransferase I retains the N‐terminus of the nascent protein in rat liver
Author(s) -
Kolodziej Marian P.,
Zammit Victor A.
Publication year - 1993
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(93)81007-m
Subject(s) - n terminus , carnitine palmitoyltransferase i , biochemistry , peptide sequence , carnitine o palmitoyltransferase , amino acid , protease , biology , peptide , c terminus , complementary dna , inner mitochondrial membrane , mitochondrion , chemistry , enzyme , gene , beta oxidation
Carnitine palmitoyltransferase I was isolated from octylglucoside extracts of rat liver mitochondrial outer membranes. This native enzyme was digested proteolytically with V8 protease. Five major peptides were obtained all of which were found in the amino acid sequence predicted from the full‐length cDNA sequence of the protein. One peptide was found to correspond to the extreme N‐terminus of the deduced amino acid sequence. Therefore, the mature protein retains the N‐terminus of the nascent protein after import into the mitochondrial membrane. Knowledge of the identity of the N‐terminus of the mature protein allows a reappraisal of the role of the two main, N‐terminal hydrophobic domains of the protein and of the possible topology of the protein within the membrane.