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Osteoblast‐like cells have a variable mixed population of purino/nucleotide receptors
Author(s) -
Yu Hesheng,
Ferrier Jack
Publication year - 1993
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(93)80994-6
Subject(s) - extracellular , nucleotide , intracellular , adenosine triphosphate , receptor , population , biology , adenosine , uridine triphosphate , biophysics , purinergic receptor , microbiology and biotechnology , biochemistry , medicine , environmental health , gene
Osteoblast‐like UMR 106.06 cells respond to extracellular application of nucleotides with a fast intracellular calcium pulse (latency of about 20 s, half‐width of about 10 s), as measured with fluo‐3 on a confocal laser scanning system. Cross‐inhibition experiments at 50 μM show that, on a cell population basis, adenosine triphosphate (ATP) strongly inhibits the effect of uridine triphosphate (UTP) or 2‐methylthio‐ATP (2‐MeSATP) applied within 2 min after the end of the ATP‐induced pulse, while prior application of UTP or 2‐MeSATP only weakly inhibits the ATP effect, and UTP and 2‐MeSATP weakly inhibit each other. Furthermore, there are clear differences in cross‐inhibition responses between individual cells. Our measurements provide strong evidence that these cells have at least two types of purino/nucleotide receptors, probably P 2y and P 2u , with a proportion that varies between individual cells.