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Translation and fates of the gag protein of 1731, a Drosophila melanogaster retrotransposon
Author(s) -
Kim Myeong-Hee,
Coulondre Christine,
Champion Serge,
Lacoste Jérôme,
Best-Belpomme Martin,
Maisonhaute Claude
Publication year - 1993
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(93)80989-8
Subject(s) - retrotransposon , drosophila melanogaster , biology , drosophila virilis , group specific antigen , nuclear localization sequence , gene , translation (biology) , genome , reporter gene , microbiology and biotechnology , genetics , virology , transposable element , gene expression , messenger rna
An entire copy of 1731, a Drosophila melanogaster retrotransposon, was tagged by fusing in frame its putative gag gene with the reporter LacZ sequence. The high transfection efficiency of Drosophila virilis cells added to the absence of 1731 in their genome allowed, by combining histochemical staining and immunological detections, the demonstration of the translation of the 1731 gag gene. The gag protein is gathered in virus‐like particles. Its occurrence in nuclei is consistent with a nuclear localization signal. The expression of the sense construction was inhibited by cotransfections with its antisense homologue.