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Phosphatidic acid induces the respiratory burst of electropermeabilized human neutrophils by acting on a downstream step of protein kinase C
Author(s) -
Mitsuyama Takashi,
Takeshige Koichiro,
Minakami Shigeki
Publication year - 1993
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(93)80967-y
Subject(s) - protein kinase c , staurosporine , calphostin c , calphostin , nadph oxidase , diacylglycerol kinase , phosphatidic acid , respiratory burst , superoxide , phorbol , bisindolylmaleimide , chemistry , biochemistry , microbiology and biotechnology , intracellular , reactive oxygen species , kinase , biology , enzyme , phospholipid , membrane
Phosphatidic acid (PA) dose‐dependently induced Superoxide (O 2 − ) production of electropermeabilized human neutrophils but not of intact neutrophils, indicating that PA induces the activation of NADPH oxidase by acting on an intracellular target. The O 2 − production by PA was not inhibited by protein kinase C (PKC) inhibitors, such as staurosporine and calphostin C, and an inhibitor of PA phosphohydrolase, propranolol. These observations suggest that the activation of the oxidase by PA is independent of the activity of PKC and may dominate the activation by diacylglycerol which is formed from PA via the action of PA phosphohydrolase. Furthermore, the production by PA, as well as that by phorbol myristate acetate, was inhibited by cyclic AMP and GDPßS. Therefore, PA seems to act at a site downstream of PKC.