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Monensin and forskolin inhibit the transcription rate of sucrase‐isomaltase but not the stability of its mRNA in Caco‐2 cells
Author(s) -
Chantret I.,
Lacasa M.,
Chevalier G.,
Swallow D.,
Rousset M.
Publication year - 1993
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(93)80964-v
Subject(s) - monensin , forskolin , messenger rna , sucrase , transcription (linguistics) , chemistry , endocrinology , medicine , gene expression , caco 2 , biology , microbiology and biotechnology , enzyme , biochemistry , gene , cell , stimulation , linguistics , philosophy
Treatment of Caco‐2 cells with forskolin (25 μM) or monensin (1 μM) has previously been shown to cause a marked decrease in the level of sucrase‐isomaltase (SI) mRNA, without any effect on the expression of dipeptidylpeptidase IV (DPP‐IV). In the present work, we report that there is no significant difference in the stability of SI mRNA between control and treated cells. On the other hand, we demonstrate a decrease in the transcription rate of SI mRNA which is sufficient to account for the decrease in the steady‐state level of SI mRNA both in forskolin‐ and monensin‐treated Caco‐2 cells.