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A putative transcription factor binding to the upstream region of the puf operon in Rhodobacter sphaeroides
Author(s) -
Shimada Hiroshi,
Ohta Hiroyuki,
Masuda Tatsuru,
Shioi Yuzo,
Takamiya Ken-ichiro
Publication year - 1993
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(93)80961-s
Subject(s) - rhodobacter sphaeroides , operon , repressor , gal operon , microbiology and biotechnology , chemistry , dna , transcription factor , binding site , biology , biochemistry , biophysics , gene , escherichia coli , photosynthesis
Gel shift assays of the upstream region of the puf operon in Rhodobacter sphaeroides were performed using cell‐free extracts from cells grown under various culture conditions. The results suggested that a protein binding to the upstream region functioned as a repressor‐like substance of the expression of the operon by oxygen tension or light. The density of the shifted band of cell‐free extracts from cells irradiated with blue light under semi‐aerobic conditions was higher than that with red light. Phosphatase treatment of the cell‐free extracts strongly increased the DNA‐binding affinity of the protein.