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Spleen‐derived growth factor, SDGF‐3, is identified as keratinocyte growth factor (KGF)
Author(s) -
Suzuki Masashi,
Itoh Takehiro,
Osada Hiroyuki,
Rubin Jeffrey S.,
Aaronson Stuart A.,
Suzuki Tohru,
Koga Nobumitsu,
Saito Takao,
Mitsui Youji
Publication year - 1993
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(93)80956-u
Subject(s) - keratinocyte growth factor , growth factor , spleen , factor (programming language) , microbiology and biotechnology , biology , cancer research , immunology , computer science , genetics , programming language , receptor
A heparin‐binding mitogen rat for rat hepatocytes was partially purified from bovine spleen by a combination of heparin‐affinity, cation‐exchange and gel‐filtration chromatography. Besides stimulating rat hepatocytes, this factor, which was designated spleen‐derived growth factor‐3 (SDGF‐3), exhibited mitogenic activity for mouse epidermal keratinocytes but not mouse fibroblasts. Its apparent epithelial specificity and heparin‐binding properties corresponded to those of keratinocyte growth factor (KGF). These findings, together with the fact that the mitogenic activity of SDGF‐3 was abolished by a neutralizing monoclonal antibody specific for KGF, identify this bovine spleen‐derived hepatocyte mitogen as KGF.