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Transcription and translation of inducible nitric oxide synthase in the pancreas of prediabetic BB rats
Author(s) -
Kleemann Rainer,
Rothe Helga,
Kolb-Bachofen Victoria,
Xie Qiao-wen,
Nathan Carl,
Martin Stephan,
Kolb Hubert
Publication year - 1993
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(93)80954-s
Subject(s) - nitric oxide synthase , transcription (linguistics) , chemistry , pancreas , translation (biology) , endocrinology , messenger rna , microbiology and biotechnology , biochemistry , medicine , enzyme , gene , biology , philosophy , linguistics
The inducible NO synthase (iNOS) was found to be expressed in pancreatic lesions of adult diabetes‐prone BB rats. Pancreatic iNOS mRNA was detected by reverse transcriptase PCR in pancreatic RNA of adult diabetes‐prone BB rats but not in normal Wistar rats, young diabetes‐prone BB rats without insulitis or in diabetes‐resistant BB rats. Immunohistochemistry of pancreatic sections using an iNOS‐specific antiserum labeled the pancreas of adult diabetes‐prone BB rats but not Wistar rats. Parallel staining for EDI‐positive macrophages showed restriction of iNOS expression to areas of islet infiltration by macrophages. In conclusion, the data provide direct evidence for enhanced expression of inducible NO synthase in tissue lesions during the development of autoimmune diabetes.