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Cis ‐acting elements in the promoter region of the human aldolase C gene
Author(s) -
Buono Pasqualina,
de Conciliis Lisa,
Olivetta Eleonora,
Izzo Paola,
Salvatore Francesco
Publication year - 1993
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(93)80936-o
Subject(s) - footprinting , gene , aldolase a , microbiology and biotechnology , palindromic sequence , biology , reporter gene , promoter , dna footprinting , transcription (linguistics) , regulatory sequence , transcription factor , genetics , palindrome , gene expression , enzyme , genome , biochemistry , linguistics , philosophy
We investigated the cis ‐acting sequences involved in the expression of the human aldolase C gene by transient transfections into human neuroblastoma cells (SKNBE). We demonstrate that 420 bp of the 5'‐flanking DNA direct at high efficiency the transcription of the CAT reporter gene. A deletion between − 420 bp and − 164 bp causes a 60% decrease of CAT activity. Gel shift and DNase I footprinting analyses revealed four protected elements: A, B, C and D. Competition analyses indicate that Spl or factors sharing a similar sequence specificity bind to elements A and B, but not to elements C and D. Sequence analysis shows a half palindromic ERE motif (GGTCA), in elements B and D. Region D binds a transactivating factor which appears also essential to stabilize the initiation complex.