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cDNA cloning and pharmacological characterization of an opioid receptor with high affinities for κ‐subtype‐selective ligands
Author(s) -
Nishi Miyuki,
Takeshima Hiroshi,
Fukuda Kazuhiko,
Kato Shigehisa,
Mori Kenjiro
Publication year - 1993
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(93)80923-i
Subject(s) - affinities , cloning (programming) , chemistry , opioid receptor , complementary dna , receptor , opioid , stereochemistry , microbiology and biotechnology , pharmacology , biochemistry , biology , gene , computer science , programming language
The amino acid sequence of a rat opioid receptor, designated as ROR‐D, has been deduced by cloning and sequencing the cDNA. The ROR‐D expressed from the cDNA exhibits high affinities for ligands selective for the opioid receptor κ‐subtype and low affinities for ligands selective for the δ‐ and μ‐subtypes. RNA blot hybridization analysis indicated that ROR‐D mRNA is distributed in cerebrum and brainstem but not in cerebellum.