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Efficient processing and export of human growth hormone by heat labile enterotoxin chain B signal sequence
Author(s) -
Ghorpade Anuja,
Garg Lalit C.
Publication year - 1993
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(93)80920-p
Subject(s) - periplasmic space , signal peptide , enterotoxin , complementary dna , escherichia coli , biochemistry , chemistry , biology , microbiology and biotechnology , peptide sequence , gene
The heat‐labile enterotoxin chain B (LTB) signal sequence was used for the processing and export of human growth hormone (hGH). The protein was completely processed and exported across the cell membrane to accumulate in the periplasmic space in Escherichia coli . The human growth hormone cDNA was cloned as a PCR amplified fragment under the control of tac promoter and translationally fused to the LTB signal sequence. The rate of processing of hGH under the control of the LTB signal sequence was equal to or more than the rate of induction of expression, indicating efficient processing. The receptor binding activity of the processed periplasmic protein was established in a radio receptor assay.