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Tyrphostin‐induced differentiation of mouse erythroleukemia cells
Author(s) -
Anafi Mordechai,
Gazit Aviv,
Gilon Chaim,
Neriah Yi Ben,
Levitzki Alexander
Publication year - 1993
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(93)80884-w
Subject(s) - phosphorylation , tyrosine phosphorylation , tyrosine , cellular differentiation , tyrosine kinase , microbiology and biotechnology , biology , kinase , receptor tyrosine kinase , protein tyrosine phosphatase , chemistry , biochemistry , signal transduction , gene
Inhibitors of protein‐tyrosine kinases (TPKs) from the tyrphostins family induce terminal erythroid differentiation of mouse erythroleukemia (MEL) cells. The most potent tyrphostin was found to be AG‐568 which was therefore investigated in more detail. Just prior to differentiation the inhibition of tyrosine phosphorylation of a pp 97 protein band was noted. We also found that AG‐568 treatment induces the appearance of a putative differentiation factor which could induce tyrphostin‐independent differentiation in untreated cells. Our study suggests that the inhibition of tyrosine phosphorylation by AG‐568 leads to the production of differentiating factor(s) which induce the MEL cells to differentiate.