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Cyclin A‐mediated inhibition of intra‐Golgi transport requires p34 cdc2
Author(s) -
Mackay Deborah,
Kieckbusch Regina,
Adamczewski Jörg,
Warren Graham
Publication year - 1993
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(93)80874-t
Subject(s) - golgi apparatus , chemistry , microbiology and biotechnology , cyclin dependent kinase 1 , biophysics , biochemistry , cell cycle , biology , cell
An in vitro assay was used to study the role of p34 cdc2 in cyclin A‐mediated vesicular transport inhibition. It was shown that the S‐phase kinase p33 cdk2 reduced the effect of cyclin A on transport assays performed with sHeLa cytosol, even though histone kinase was strongly activated. Also, transport with FT210 cytosol (which is temperature‐sensitive for p34 cdc2 ) was inhibited by cyclin A only at the permissive temperature. However, the phosphatase inhibitor microcystin inhibited transport without any requirement for p34 cdc2 activity. These results show that transport is inhibited by cyclin A via p34 cdc2 , and also by another kinase, possibly downstream of p34 cdc2 .