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Long‐term regulation of pyruvate dehydrogenase kinase by high‐fat feeding
Author(s) -
Orfali Karen A.,
Fryer Lee G.D.,
Holness Mark J.,
Sugden Mary C.
Publication year - 1993
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(93)80864-q
Subject(s) - medicine , nefa , endocrinology , pyruvate kinase , pyruvate dehydrogenase complex , insulin , calorie , chemistry , kinase , metabolism , biology , enzyme , biochemistry , glycolysis
The provision of the high‐fat diet (47% of calories as fat) for 28 days evoked a significant decline in cardiac PDH a , activity, together with marked increases in the activity of PDH kinase measured in isolated mitochondria and freshly‐prepared cardiomyocytes from adult rats. Plasma insulin concentrations in fat‐fed rats were not significantly different from control, but plasma NEFA concentrations were elevated. PDH kinase activity in cardiomyocytes from fat‐fed rats fell substantially in culture (25 h). This decline was prevented by the inclusion of n ‐octanoate and DBcAMP in combination, but not individually, in the culture medium. The results are discussed in relation to the role for fatty acids and insulin in the long‐term modulation of cardiac PDH kinase activity by high‐fat feeding.