Premium
Organisation of the chicken annexin V gene and its correlation with the tertiary structure of the protein
Author(s) -
Pfannmüller Eva,
Turnay Javier,
Bertling Wolf,
von der Mark Klaus
Publication year - 1993
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(93)80857-q
Subject(s) - exon , gene , intron , tata box , microbiology and biotechnology , biology , promoter , annexin a2 , tandem exon duplication , coding region , genetics , homology (biology) , alternative splicing , exon trapping , annexin , gene expression , flow cytometry
Chicken annexin V (anchorin CII) is a collagen binding, membrane‐associated molecule with Ca 2+ channel activity. Here we report on the coding sequences, promoter region, size and distribution of exons, and exon‐intron junctions of the chicken annexin V gene. It is about 25 kb long and codes for 13 short exons between 50 and 581 bp length. Exon sizes and locations of splice sites are almost completely homologous to those of the human and mouse annexin II or pigeon annexin I genes, although there is only 50–60% homology in the sequence of the corresponding proteins. The four repeat structure and symmetry of the annexin V as evident from sequence and X‐ray analysis studies is only partially reflected in this highly conserved exon distribution. In the first two repeats of chicken annexin V the exons correlate with protein domains containing one, two, or three α‐helices, while in the repeats 3 and 4 exon junctions and α‐helical domains do not correlate. The analysis of the promotor structure revealed the absence of a typical TATA‐box, but a GC‐rich region which may possibly promote transcription from several start sites.