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Molecular cloning, functional expression and localization of an inward rectifier potassium channel in the mouse brain
Author(s) -
Morishige Ken-Ichirou,
Takahashi Naohiko,
Findlay Ian,
Koyama Hidekazu,
Zanelli Jill S.,
Peterson Christine,
Jenkins Nancy A.,
Copeland Neal G.,
Mori Nozomu,
Kurach Yoshihisa
Publication year - 1993
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(93)80840-q
Subject(s) - inward rectifier potassium ion channel , complementary dna , microbiology and biotechnology , in situ hybridization , cdna library , biology , xenopus , molecular cloning , cloning (programming) , clone (java method) , expression cloning , potassium channel , gene , gene expression , genetics , ion channel , biophysics , receptor , computer science , programming language
We have cloned an inward‐rectifier potassium channel from a mouse brain cDNA library, studied its distribution in the brain by in situ hybridization and determined the chromosomal localization of the gene. A mouse brain cDNA library was screened using a fragment of the mouse macrophage IRK1 cDNA as a probe. Two duplicate clones of ~5.5 kb were obtained. Xenopus ococytes injected with cRNA derived from the clone expressed a potassium channel with inwardly rectifying channel characteristics. The amino acid sequence of the clone was identical to that of IRK1 recently cloned from a mouse macrophage cell line. In situ hybridization study showed the mouse brain IRK1 to be generally distributed throughout the brain, but in particular subsets of neurons at high levels. The gene was placed in the distal region of mouse chromosome 11, which contains several uncloned neurological mutations. These results provide the first demonstration of the cloning and distribution of an inward rectifier potassium channel from the nervous system.