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Identification of DNA binding proteins in vaccinia virus by DNA‐protein crosslinking
Author(s) -
Chkheidze A.N.,
Prikhod'ko G.G.,
Karpov V.L.,
Vassilenko S.K.,
Mirzabekov A.D.
Publication year - 1993
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(93)80833-g
Subject(s) - dna , microbiology and biotechnology , vaccinia , gel electrophoresis , virus , gene , dna binding protein , biology , single stranded binding protein , promoter , dna sequencing , virology , chemistry , recombinant dna , biochemistry , gene expression , transcription factor
DNA binding proteins of vaccinia virus (VV) virions, strain LIVP, have been studied by their covalent crosslinking to DNA, using two‐dimensional gel retardation electrophoresis of crosslinked DNA and proteins as well as the ‘protein image’ hybridization assay. Five proteins with molecular masses of 16, 25, 27, 41 and 54 kDa, respectively, associated with all analysed DNA sequences, including early and late genes and their promoters, have been identified.