The unusual  rps3 ‐like orf712 is functionally essential and structurally conserved in  Chlamydomonas | Zendy

Liu Xiang-Qin | Zendy; Huang Changzhi | Zendy; Xu Hui | Zendy

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The unusual rps3 ‐like orf712 is functionally essential and structurally conserved in Chlamydomonas

Author(s) -

Liu Xiang-Qin,

Huang Changzhi,

Xu Hui

Publication year - 1993

Publication title -

febs letters

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.593

H-Index - 257

eISSN - 1873-3468

pISSN - 0014-5793

DOI - 10.1016/0014-5793(93)80808-8

Subject(s) - chlamydomonas reinhardtii , chlamydomonas , ribosomal protein , gene , biology , open reading frame , genetics , chloroplast , conserved sequence , peptide sequence , ribosome , rna , mutant

The Chlamydomonas reinhardtii chloroplast orf712 is a previously described open reading frame that lacks a detectable transcript but potentially encodes a polypeptide with sequence similarities to ribosomal protein Rps3 only at its N‐ and C‐tennini. Here we report that orf712 is an essential gene, as demonstrated through gene disruption by particle gun‐mediated chloroplast transformation. We also show that an orf712 is present and structurally conserved in all of the two or three major Chlamydomonas lineages. Our results suggest that orf712 is an unusual rps3 gene that contains a large translated intervening sequence.

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