Premium
The presence of a calcium‐sensitive p26‐containing complex in bovine retina rod cells
Author(s) -
Gorodovikova Ele.,
Philippov Pavel P.
Publication year - 1993
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(93)80746-h
Subject(s) - egta , recoverin , calcium , dissociation (chemistry) , homogeneous , chemistry , retina , biochemistry , biophysics , biology , retinal , rhodopsin , physics , organic chemistry , neuroscience , thermodynamics
A protein with apparent M r , 26 kDa (p26 [(1991) Biokhnya 56, 225‐228] or recoverin [(1991) Science 251, 915‐918]) was suggested to activate GC in a calcium dependent manner in bovine retina rod cells [(1991) Science 251, 915‐918; (1991) EMBO J, 10, 793‐798]. However, according to our present data homogeneous p26 preparations do not activate the enzyme. At the same time we have revealed a complex of p26 with an unidentified protein, presumably RK, in bovine ROS. Calcium favours formation of the complex whereas EGTA addition (which corresponds to a low free Ca 2+ concentration) leads to its dissociation.