Premium
Different factors bind to the regulatory region of the Na + ,K + ‐ATPase α1‐subunit gene during the cell cycle
Author(s) -
Kawakami Kiyoshi,
Yanagisawa Ken,
Watanabe Yuko,
Tominaga Shin-ichi,
Nagano Kei
Publication year - 1993
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(93)80740-l
Subject(s) - cell cycle , protein subunit , gene expression , gene , atpase , microbiology and biotechnology , transcription (linguistics) , stimulation , chemistry , cell , transcription factor , biology , mutant , regulation of gene expression , biochemistry , enzyme , linguistics , philosophy , neuroscience
Three factors that bind to the positive regulatory region (ARE) of the Na + ,K + ‐ATPase α1‐subunit gene were shown to be present in growing BALB/c‐3T3 cells as shown by the gel retardation assay pattern in which three specific complexes (C1, C2 and C3) were identified. The complexes are similar to those observed in MDCK cell nuclear extracts in which linker substitution mutations in the competitor gave parallel specific effects in both cells. During the process of the cell growth cycle, the relative mobility of C3 was altered, and the amount of C1 decreased in the G 0 state. All three complexes (C1, C2 and C3) disappeared and other specific complexes with higher mobilities were alternatively observed at 6h after serum stimulation and thereafter. The expression of the mRNA for the α1‐subunit gene was repressed at G 0 and gradually increased after serum stimulation. These results suggest that different sets of factors are responsible for the transcription of the gene at different stages of the cell cycle.