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Detection of Duchenne muscular dystrophy gene products in amniotic fluid and chorionic villus sampling cells
Author(s) -
Prigojin Hagit,
Brusel Marina,
Fuchs Ora,
Shomrat Ruth,
Legum Cyril,
Nudel Uri,
Yaffe David
Publication year - 1993
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(93)80734-c
Subject(s) - chorionic villus sampling , duchenne muscular dystrophy , amniotic fluid , prenatal diagnosis , dystrophin , microbiology and biotechnology , chorionic villi , western blot , muscular dystrophy , biology , fetus , andrology , gene , gene expression , messenger rna , medicine , pregnancy , genetics
We have examined the expression of several Duchenne muscular dystrophy (DMD) gene products in amniotic fluid (AF) and chorionic villus sampling (CVS) cells. Variable amounts ofdystrophin could be detected in most CVS and AF samples by immunoprecipitation followed by Western blot analysis. PCR analysis demonstrated the presence of the muscle type dystrophin mRNA in all AF cell cultures. The brain type dystrophin mRNA was also detected in some of these cultures. These DMD gene transcripts are of fetal origin and are produced by most or all clonable AF cells. The results may facilitate the development of a method for prenatal diagnosis of DMD, based on the expression of the gene in AF and CVS cells.