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Secretagogue‐induced calcium wave shows higher and prolonged transients of nuclear calcium concentration in mast cells
Author(s) -
Katagiri Satoshi,
Takamatsu Tetsuro,
Minamikawa Tetsuhiro,
Fujita Setsuya
Publication year - 1993
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(93)80708-3
Subject(s) - cytoplasm , secretagogue , calcium , degranulation , nucleus , chemistry , biophysics , mast cell , microbiology and biotechnology , biology , biochemistry , immunology , receptor , organic chemistry
To clarify the mechanism of secretagogue (compound 40/80)‐induced calcium signaling in rat peritoneal mast cells, we analyzed serial confocal calcium images with high spatial and temporal resolution using different Ca 2+ ‐probes. The Ca 2+ ‐wave began at the periphery of the cytoplasm, and then spread to the center of the nucleus. Nuclear [Ca 2+ ] i was clearly higher than cytoplasmic [Ca 2+ ] i . The heterogeneity of [Ca 2+ ] i continued until about 2 min after degranulation. These results suggest the existence of an intranuclear Ca 2+ ‐store which possesses a Ca 2+ ‐releasing mechanism similar to that in the cytoplasm.