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Activation of NF‐κB by cAMP in human myeloid cells
Author(s) -
Serkkola Elina,
Hurme Mikko
Publication year - 1993
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(93)80704-x
Subject(s) - transcription factor , electrophoretic mobility shift assay , nfkb1 , microbiology and biotechnology , kappa , nf κb , intracellular , dna , myeloid , chemistry , reporter gene , biology , gene , gene expression , signal transduction , biochemistry , cancer research , linguistics , philosophy
Nuclear factor kappa B (NF‐κB) is a DNA‐binding regulatory factor that controls the transcription of a number of genes. Various agents are known to activate this factor. We have studied the ability of cAMP to stimulate NF‐κB activity in human myeloid cells. Electrophoretic mobility assay revealed that structural cAMP analogs and agents elevating intracellular cAMP levels induced NF‐κB DNA‐binding activity. The inducibility was dependent on the maturation stage of myeloid cells. In promyelocytic HL‐60 cells cAMP induced higher NF‐κB activity than in more differential THP‐1 cells and in human monocytes. By transfecting HL‐60 and THP‐1 cells with reporter constructs containing NF‐κB DNA‐binding sites, we observed that cAMP‐induced NF‐κB was transcriptionally active.