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Transcription in vitro promoted by the Agrobacterium VirG protein
Author(s) -
Endoh Hideki,
Aoyama Takashi,
Oka Atsuhiro
Publication year - 1993
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(93)80694-p
Subject(s) - chemistry , in vitro , transcription (linguistics) , microbiology and biotechnology , biochemistry , biology , philosophy , linguistics
Expression of the virulence genes ( vir ) on the hairy‐root‐inducing plasmid pRiA4 is induced by plant signals in Agrobacterium cells through a two‐component regulatory system, the VirA‐VirG system. We constructed an in vitro transcription system that consisted of the purified VirG protein and the Agrobacterium RNA polymerase holoenzyme. Both versions of VirG, the non‐phosphorylated form and the VirA‐phosphorylated form, were active but showed different patterns of the pH‐dependency for transcriptional activation.