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Interleukin‐1β‐induced cytosolic phospholipase A 2 activity and protein synthesis is blocked by dexamethasone in rat mesangial cells
Author(s) -
Schalkwijk Casper G.,
Vervoordeldonk Margriet,
Pfeilschifter J.,
van den Bosch Henk
Publication year - 1993
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(93)80683-l
Subject(s) - cytosol , dexamethasone , chemistry , mesangial cell , endocrinology , medicine , microbiology and biotechnology , biochemistry , biology , enzyme , in vitro
Interleukin‐1β induces gene expression and secretion of the secretory phospholipase A 2 (sPLA 2 ) and prostaglandin E 2 (PGE 2 ) release from rat mesangial cells. We have previousy shown that prolonged treatment of rat mesangial cells with interleukin‐1β (IL‐1β) also enhances the cytosolic phospholipase A; (cPLA 2 ) activity. This effect of IL‐1β on the cPLA 2 activity is inhibited by actinomycin D and cycloheximide, indicating that both transcription and translation are involved. Here, we describe that IL‐1β increases mRNA levels and protein synthesis of cPLA 2 itself. In parallel with the effect of dexamethasone on the sPLA 2 , this glucocorticoid inhibits the IL‐1β‐enhanced cPLA 2 activity as a result of the suppression of IL‐1β‐induced cPLA 2 gene expression. This report suggests that the pro‐inflammatory action of interleukin‐1β may, in part, be mediated by its effects on cPLA 2 activity.